The US Embassy in Ukraine's capital Kyiv was temporarily closed on Wednesday, as the mission received information of a "potential significant air attack."
The move comes a day after Ukraine launched US long-range missiles into Russian territory for the first time on Tuesday, using ATACMS missiles against a facility in the Bryansk region close to the border.
Russia warned on Tuesday that it would respond.
A statement released by the Embassy advised employees to shelter in place and "recommends US citizens be prepared to immediately shelter in the event an air alert is announced."
Further details regarding the potential attack have not been disclosed.
Russia had been warning the West for months that if Washington allowed Ukraine to fire US, British and French missiles deep into Russia, Moscow would consider those NATO members to be directly involved in the war in Ukraine.
On Tuesday, Putin lowered the threshold for a nuclear strike in response to a broader range of conventional attacks, with nuclear risks rising amid the highest tensions between Russia and the West in more than half a century.
US President Donald Trump has responded with a swift rejection of Iran's response to a US peace proposal on Sunday that included ending the war on all fronts, including Lebanon, providing compensation for war damages, and emphasising Iranian sovereignty over the Strait of Hormuz.
Iran has sent its response to a US proposal for peace talks to end the war, Iranian state media reported on Sunday, as two ships were allowed to pass through the blockaded Strait of Hormuz.
Groups of passengers and crew disembarked from a cruise ship hit by a deadly hantavirus outbreak on Sunday to be evacuated to their home countries where they will isolate according to national protocols to prevent further spread of the disease.
Israeli strikes killed at least three Palestinians in Gaza on Sunday, including two members of the Hamas-run police force, health officials said, in violence that underscored the fragility of a US-brokered ceasefire.